Early detection of acute myocardial infarction with the new marker Fatty Acid-Binding Protein : rapid testing and diagnostic value

Abstract

There are several requirements for the early detection of AMI with biochemical markers: The biochemical marker should be: - Specific - Sensitive - Fast elevated after the clinical onset of the symptoms. The test should be: - Easy to perform - Give a fast result - Preferably bedside - Inexpensive Here, we demonstrate the biochemical characteristics of heart-type Fatty Acid-Binding Protein (FABP) in AMI and UAP patients. Also, we show some preliminary data on a new in-house made quantitative rapid test with simple test procedures. - Patients: 50 patients of the CCU of PWH are included in the study diagnosed with UAP or AMI. Eight blood samples per patient were taken at 0-72 hours after admission and analyzed with a laboratory ELISA test. - Rapid test procedure: 80 ml plasma or serum is added on the sample pad. Within 5 minutes, the strip can be read out with an optical reader (the PART from LRE, Munich, Germany). A release curve for AMI patients shows a significantly elevated FABP concentration already 1 hour after infarction, whereas for CPK, this is found after 3 hours. Comparing the ROC curve for patients arriving at the hospital and 1 hour later for FABP and CPK demonstrates the preference of FABP over CPK. The preliminary results of the rapid test show for selected plasma and serum samples a measuring range of 0-200 ng/ml FABP. The availability of a rapid bedside test for FABP enables faster diagnosis in patients with no clear ECG. This can be used for both inclusion or exclusion of AMI.